Use 1 group for the thermostat
@@ -12,7 +12,7 @@ comm-grps = system ; Remove center of mass motion of system
################################## ENSEMBLE ####################################
ref_t = 300 ; System temperature (K)
tau_t = 1.0 ; Thermostat time constant (ps)
-tc_grps = Protein Non-Protein ; Apply thermostat to system
+tc_grps = System ; Apply thermostat to whole system
continuation = yes
############################ BONDED INTERACTIONS ###############################
constraints = hbonds ; Constrain bonds to hydrogen atoms
